Production hyaluronic acid with resting cell of group A streptococcus hemolyticus
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چکیده
منابع مشابه
The biosynthesis of hyaluronic acid by group A streptococcus.
Previous publications from this laboratory have reported the use of a strain of group A hemolytic streptococcus for the study of the biosynthesis of the mucopolysaccharide, hyaluronic acid (HA) (1, 2). Methods were devised for the isolation of HA of high purity and the isolation and degradation of fragments of the molecule. Glucose-l-C4 and glucose-6-C4 were used to demonstrate that both the gl...
متن کاملStreptococcus Hemolyticus: A Study of Virulence *
In 1928 Duran-Reynals8 reported that vaccine virus skin infections in rabbits were intensified when the virus used was extracted from testicular tissue. This subject was rapidly developed and the agent responsible came to be designated "spreading factor." Spreading factor was reported to be present not only in organ extracts of mammalian testes, but also to a lesser degree in the kidney and ski...
متن کاملThe biosynthesis of hyaluronic acid by Streptococcus.
The mechanism of hyaluronic acid chain growth in a particulate enzyme preparation obtained from Group A Strepfococcus has been examined and additional characteristics of the enzyme detailed. Hyaluronic acid chain elongation occurs by the transfer of monosaccharide units from uridine nucleoside diphosphate derivatives to the nonreducing ends of endogenous oligosaccharide. This oligosaccharide wh...
متن کاملBiosynthesis of hyaluronic acid by Streptococcus.
Synthesis of hyaluronic acid was investigated in a cell-free system derived from a strain of Group A streptococci. Preparative procedures were improved so that an enzyme system 70 times more active than that previously reported was obtained. The hyaluronic acid synthesized could be separated into trichloroacetic acid-soluble and -insoluble fractions. On the basis of pulse-chase experiments, it ...
متن کاملSynthesis of capsular polysaccharide (hyaluronic acid) by protoplastmembrane preparations of group A Streptococcus.
Previous reports from this laboratory demonstrated the synthesis of hyaluronic acid by a cell-free preparation obtained from a strain of Group A streptococcus. The enzymic activity which sedimented at 105,000 x g, but not at 10,000 x g, required the addition of uridine diphospho-N-acetylglucosamine, uridine diphosphoglucuronic acid, and Mg++ for the formation of hyaluronic acid (l-3). Since hya...
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ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1955
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.10.269